Research Paper
Year: 2023 | Month: May | Volume: 10 | Issue: 5 | Pages: 244-251
DOI: https://doi.org/10.52403/ijrr.20230530
Structural Prediction of Melanocortin Receptor (MC4R) of Carassius Auratus: An In-silico Approach
Samik Acharjee
Department of Zoology, Rammohan College, Kolkata-700009; West Bengal, India
ABSTRACT
Carassius auratus commonly known as goldfish is one of most attractive fish used as ornamental fishes throughout the world. From the ancient times, the melanocortin system is considered to be conserved ranging from teleosts to mammals. Various researches in several fish species presented melanocortin receptors 4 (MC4R) is involved in different functions in fish body. Therefore, in-silico analysis of MC4R of Carassius auratus has been carried out in the study to know the physicochemical properties and 3D structural confirmation of the protein. Sequence containing accession number XM_026207258.1 was taken from National Center for Biotechnology Information (NCBI) and then processed to Expasy’s protparam for the physicochemical characterization, SOPMA for secondary structure prediction. Template search was done by SWISSMODEL/Workspace and Swiss-Pdb-Viewer was used for prediction of 3D structure of the concerned protein. The results suggested MC4R is a stable, hydrophobic and slightly basic nature of protein. The secondary structure of the analysed MC4R protein suggested presence of 50.77% alpha helix, 19.69% extended strands and 3.38% beta turns along with 26.15% random coils. Moreover, it might be resolved from the ramachandran plot that the structural prediction of MC4R is correct in prediction. Further, predicted 3D structure of MC4R protein can also be utilized for docking and simulation studies in future for finding out its relative binding affinity and functional action mechanism aspects. Moreover, the study is an approach for reducing the sequence data and solved structures gap by X-ray crystallography and NMR spectroscopy, which are also tedious and expensive laboratory techniques in application as well.
Keywords: Melanocortin receptors 4 (MC4R); In silico analysis; 3D structural prediction; Carassius auratus.
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