Original Research Article
Year: 2023 | Month: July | Volume: 10 | Issue: 7 | Pages: 12-18
DOI: https://doi.org/10.52403/ijrr.20230703
Oral Administration of Ethanol Extract of Moringa Leaves (Moringa oleifera) Reduces F2-Isoprostane and Monocyte Chemoattractant Protein (MCP-1) Levels in Wistar Rats (Rattus norvegicus) as an Obesity Model
Stefani Krisanti1, Agus Eka Darwinata2, I Made Jawi3
11Anti-Aging Medicine Post Graduate Biomedic Program Faculty of Medicine, Udayana University
2Clinical Microbiology Department Faculty of Medicine, Udayana University,
3Pharmacology Department, Faculty of Medicine, Udayana University, Denpasar, Indonesia.
Corresponding Author: Stefani Krisanti
ABSTRACT
Background: Obesity is a chronic condition causes oxidative stress and low-grade chronic inflammation characterized by increased oxidative stress biomarkers F2-Isoprostane and proinflammatory biomarkers Monocyte Chemoattractant Protein (MCP-1). Oxidative stress and inflammation accelerate aging process. Moringa leaves (Moringa oleifera) are known as one of the antioxidant-rich plants containing polyphenols, flavonoids, saponin and tannin which have strong antioxidant and anti-inflammatory effects. This study was conducted to prove oral administration of ethanol extract of Moringa leaves in reducing F2-Isoprostane and MCP-1 levels in Wistar rats (Rattus norvegicus) as an obesity model.
Methods: This study was a pure experimental study using a Randomized Pretest-Posttest Control Group Design. Thirty Wistar rats, male, aged 3.5-4.5 months used in this study. Twenty rats were induced obesity with high-fat-diet until reached Lee's Index ≥ 0.30 g/cm, then 30 rats were divided into three groups, each consisting of 10 rats, P0 group was given normal diet, P1 group was given high-fat-diet and aquadest 1cc/day/rat per sonde and P2 group was given high-fat-diet and moringa leaf extract doses 300mg/kgBW/rat/day per sonde. Obesity induction was carried out for 32 days and treatment was given for 32 days. F2-Isoprostane and MCP-1 levels were measured before and after treatment.
Results: Serum F2-Isoprostane and MCP-1 levels showed a significant decrease in obese rats fed with high-fat-diet and moringa leaf extract for 32 days (p<0.001). Moringa leaf extract also prevent weight gain in obese rats.
Conclusion: Moringa leaf inhibit oxidative stress and inflammatory process related to obesity and comorbidities thus expected to inhibit the aging process.
Keywords: : moringa leaf; F2-Isoprostane; MCP-1; obesity; aging.
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