Original Research Article
Year: 2023 | Month: July | Volume: 10 | Issue: 7 | Pages: 235-240
DOI: https://doi.org/10.52403/ijrr.20230730
Administration of Arabica Coffee Bean (Coffea Arabica) Cream Extract from Wamena Papua Can Reduce MMP-1 And Increase Collagen in Wistar Male Rats (Rattus Norvegicus) Exposed to Ultraviolet-B
Made Rendra Wisnu Rahasbistara1, I Gusti Nym Darmaputra2, Ni Made Linawati3, Ketut Kwartantaya Winaya2, Ida Iswari4, Dewa Ayu Agus Sri Laksemi5
1Master Program in Biomedical Science, Anti-Aging Medicine, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
2Department of Dermatovenereology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
3Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
4Department of Clinical Microbiology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
5Department of Parasitology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
Corresponding Author: Made Rendra Wisnu Rahasbistara
ABSTRACT
Introduction: The aim of the study was to determine the effectiveness of Arabica coffee bean extract cream (Coffea arabica) from Wamena in reducing MMP-1 levels and increasing collagen density in Wistar rats (Rattus norvegicus) exposed to UV-B light.
Methods: This is a post-test only control group design experimental study using male rats as subjects. Each group was exposed to UV light and smeared with coffee bean cream extract from Wamena every day. Then the back skin tissue was taken and the amount of collagen was examined by Picro Sirius Red staining and MMP1 levels were examined by ELISA staining.
Results: The mean MMP-1 levels in groups P1, P2, and P3 respectively 7.57 ± 0.70, 5.99 ± 0.60, and 4.57 ± 0.36. The average percentage of collagen in the P1, P2, and P3 groups was 72.23 ± 4.42, 77.80 ± 2.73, and 81.05 ± 2.77, respectively. The average MMP-1 level decreased with the increasing dose of Wamena arabica coffee bean extract cream. The mean percentage of collagen increased with increasing doses of Wamena arabica coffee bean extract cream in experimental animals.
Conclusion: Administration of 3% and 6% Wamena Arabica coffee bean extract cream can inhibit the increase in MMP-1 levels and can increase the amount of collagen in male wistar rats (Rattus norvegicus) exposed to UV-B light.
Keywords: Arabica coffee bean, MMP-1, Collagen
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