Year: 2024 | Month: December | Volume: 11 | Issue: 12 | Pages: 56-61
DOI: https://doi.org/10.52403/ijrr.20241206
Simultaneous Determination of Vitamins B1, B2, B3, B6, and B12 in Tablet Forms Using the Area Under the Curve Ultraviolet Spectrophotometry Method
M. Deddy Harfiansyah1, Effendy De Lux Putra2, Siti Morin Sinaga3
1,2,3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Corresponding Author: Effendy De Lux Putra
ABSTRACT
This research focuses on creating the area under the curve (AUC) ultraviolet spectrophotometry method approach to assess the concentrations of vitamins B1, B2, B3, B6, and B12 in tablet formulations, bypassing the need for separation steps. The technique relies on calculating the AUC values across different concentrations within a specified wavelength range, using methanol as the solvent. Subsequently, the method was applied to evaluate the levels of these vitamins in the tablets. The analysis targeted specific wavelength ranges for each vitamin: 250-260 nm for B1, 279-289 nm for B2, 267-277 nm for B3, 312-322 nm for B6, and 382-392 nm for B12. The average recovery rates achieved were 100.07% for B1, 100.70% for B2, 100.52% for B3, 100.02% for B6, and 99.90% for B12 in the tablet samples. This method proved effective for the simultaneous analysis of vitamins B1, B2, B3, B6, and B12 in tablet forms and satisfied the necessary validation criteria.
Keywords: Vitamins B1, B2, B3, B6, B12, the area under the curve, ultraviolet spectrophotometry.
[PDF Full Text]