Year: 2024 | Month: May | Volume: 11 | Issue: 5 | Pages: 87-92
DOI: https://doi.org/10.52403/ijrr.20240511
Antibacterial Activity by Diffusion and Dilution Methods of Liquid Smoke Cocos nucifera L.
Lusi Mardika Ariyanti3, Masfria1,2, Muchlisyam1
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
2Nanomedicine Center of Innovation, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
3Undergraduate Program, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Corresponding Author: Masfria
ABSTRACT
Introduction: Liquid smoke is a product that obtained industrially from coconut shell through pyrolysis process at 400°C for 2,5 hours and redestillation to obtain grade 1 liquid smoke. Coconut shell liquid smoke contains chemical compounds, namely phenol, which has been researched to act as a disinfectant that has broad spectrum antibacterial activity against gram positive and gram negative bacteria.
Method: This study aims to determine the potential of cocos nucifera L. liquid smoke as an antibacterial against Staphylococcus aureus and Methicillin-resistant staphylococcus aureus (MRSA) bacteria using diffusion and dilution methods. The concentrations used were 100%, 80%, 60%, 40%, 35%, 30%, 15%, 10%, 7%, 5%. As positive control was used Tetracycline 25 ppm.
Results: Liquid smoke has the potential to inhibit the growth of both bacteria. The effective concentration to inhibit the growth of S. aureus at 40% concentration and MRSA at 80% concentration. The MIC value of liquid smoke against S. aureus is 0,78%, while on MRSA 3,12%. The MBC of S. aureus and MRSA bacteria at concentrations 1,56% and 6,25%. Staphylococcus aureus has higher sensitivity to Cocos nucifera L. grade 1 liquid smoke than MRSA bacteria.
Conclusion: Cocos nucifera L. liquid smoke has antibacterial activity against Staphylococcus aureus and MRSA at effective concentrations of 40% and 80%. The MIC value of S. aureus is 0,78%, while MRSA is 3,12%. The MBC of S. aureus and MRSA bacteria at concentrations 1,56% and 6,25%.
Keywords: antibacterial, liquid smoke, phenol, Cocos nucifera L.
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