Year: 2024 | Month: August | Volume: 11 | Issue: 8 | Pages: 583-590
DOI: https://doi.org/10.52403/ijrr.20240862
Erythrosine Inhibits the Contractile Function of Duodenal Visceral Smooth Muscle of Rat Ex Vivo by Augmenting the Nitrergic Signaling Pathway
Anisha Bhowmick1, Sourapriya Mukherjee2, Goutam Paul3
Department of Physiology, Kalyani University, Kalyani, India.
Corresponding Author: Goutam Paul
ABSTRACT
Erythrosine, a poly-iodinated xanthene dye, extensively used as a dye in foods, cosmetics and pharmaceuticals. Because of its stability and bright persistent color, it is widely used as a food coloring agent in the food industry. Despite being reported of its toxicity by some researchers, it is widely used in food to attract customers. As a result, people are often exposed to Erythrosine by consuming Erythrosine contaminated food. Therefore, the aim of the study was to determine how Erythrosine affected the contractile activity and motility of the duodenum, an important component of the small intestine. To investigate the effect of Erythrosine on duodenal visceral smooth muscle contractile activity, we observed its effect on duodenal contractions by recording ex vivo duodenal movements of control and erythrosine-exposed rats using an isotonic transducer (IT 2245) connected to RMS polyrite D. Erythrosine exposed rats showed a significant suppression of the duodenal contractions through decrease in both frequency and amplitude of contractions compared to control rats in a dose-response pattern. In addition, to investigate possible neurocrine mechanisms involved in Erythrosine -induced suppression of duodenal visceral smooth muscle (dVSM) contraction in response to Erythrosine and nitrergic antagonists, duodenal movements were recorded in response to application of Erythrosine and L-NAME (nitric oxide synthase inhibitor) and Methylene blue (MB) (soluble guanylyl cyclase blocker). The degree of inhibition of dVSM contraction by Erythrosine is counteracted in L-NAME and MB pretreated conditions. Therefore, Erythrosine inhibits the dVSM contractile activity by inhibiting dVSM contractions, thereby facilitating the action of inhibitory nitrergic-myenteric efferents through the nitric oxide-mediated soluble guanylyl cyclase pathway.
Keywords: Erythrosine, contractile function, duodenal visceral smooth muscle, nitrergic-myenteric efferents, soluble guanylyl cyclase pathway.
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