Year: 2025 | Month: January | Volume: 12 | Issue: 1 | Pages: 304-311
DOI: https://doi.org/10.52403/ijrr.20250139
Estrogenic Activity of Purple Sweet Potato (Ipomoea batatas L.) Ethanol Extract on the Estrous Cycle of Wistar Rats in a Menopause Model
I Putu Bintang Pradita Kirana1, Ida Ayu Dewi Wiryanthini2, I Wayan Gede Sutadarma3, Ni Wayan Tianing2
1Undergraduate of Medicine Study Program, Faculty of Medicine, Udayana University, Bali, Indonesia,
2Department of Biochemistry, Faculty of Medicine, Udayana University, Bali, Indonesia,
3Department of Clinical Nutrition, Faculty of Medicine, Udayana University, Bali, Indonesia.
Corresponding Author: I Putu Bintang Pradita Kirana
ABSTRACT
Menopause is a condition in which menstruation ceases due to ovarian follicles no longer producing estrogen. This condition can reduce the quality of life by causing physical and psychological disturbances. While hormone therapy is commonly used, its side effects necessitate alternative approaches, such as phytoestrogens derived from purple sweet potato anthocyanins. This study evaluates the estrogenic activity of ethanol extract from purple sweet potato (Ipomoea batatas L.) on the estrous cycle of menopausal Wistar rats. A post-test-only control group design was employed using preserved biological materials. The study sample consisted of 5 rats per group, with a total of 3 groups: a negative control group (aquadest), an intervention group with ethanol extract of purple sweet potato at a dose of 400 mg/kgBW/day, and another intervention group at a dose of 600 mg/kgBW/day. Observations were conducted using a microscope to count the average number of cells in 10 fields of view. Subsequently, the estrous phases were identified to determine the duration of the estrous cycle. The data were analyzed using one-way ANOVA and Kruskal-Wallis tests. The results showed p-values for the duration of the estrous cycle, proestrus phase, estrus phase, metestrus phase, and diestrus phase as 0.289 (p>0.05), 0.368 (p>0.05), 0.310 (p>0.05), 0.174 (p>0.05), and 0.929 (p>0.05), respectively. Despite the lack of statistical significance, descriptive variations in cycle duration were observed among groups. It can be concluded that there is estrogenic activity from the ethanol extract of purple sweet potato on the estrous cycle of Wistar rats in a menopause model.
Keywords: Estrous cycle, Menopause, Phytoestrogen, Purple sweet potato
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