Year: 2025 | Month: March | Volume: 12 | Issue: 3 | Pages: 1-6
DOI: https://doi.org/10.52403/ijrr.20250301
Effects of Vitamin C and Jamblang Leaf Extract (Syzygium cumini) on Catalase Enzyme Activity in Lead Acetate-Induced Rats
Helena Azriz1, Elmatris Sy2, Rauza Sukma Rita2, Hirowati Ali2, Lili Irawati3, Zurayya Fadila4
1 Medical Doctor Study Program, Faculty of Medicine, Universitas Andalas, Limau Manis, Pauh, Padang, West Sumatera 25163, Indonesia
2Department of Biochemistry, Faculty of Medicine, Universitas Andalas, Limau Manis, Pauh, Padang, West Sumatera 25163, Indonesia
3Department of Physiology, Faculty of Medicine, Universitas Andalas, Limau Manis, Pauh, Padang, West Sumatera 25163, Indonesia
4Department of Public Health Sciences and Community Medicine, Faculty of Medicine, Universitas Andalas, Limau Manis, Pauh, Padang, West Sumatera 25163, Indonesia.
Corresponding Author: Rauza Sukma Rita
ABSTRACT
Lead is a toxic heavy metal that causes oxidative stress and suppresses endogenous antioxidants, such as the catalase enzyme. Vitamin C and jamblang leaves are exogenous antioxidants to inhibit oxidative stress. This research aimed to determine the effect of jamblang leaf extract and vitamin C on the catalase enzyme activity of mice that had been induced by acetate lead. This research was true experimental research with a randomized post-test-only control group design using mice divided into 7 groups, namely, group K- (group was given a standard diet), acetate lead 40 mg/kgBW induced K+, P1 was induced by acetate lead 40 mg/kgBW and jamblang leaves extract 75 mg/kgBW, P2 was induced by acetate lead 40 mg/kgBW and jamblang leaves extract 150 mg/kgBW, P3 was induced by acetate lead 40 mg/kgBW and vitamin C 75 mg/kgBW, P4 was induced by acetate lead 40 mg/kgBW and jamblang leaves extract 75 mg/kgBW as well as vitamin C 35 mg/kgBW, P5 was induced by acetate lead 40 mg/kgBW and jamblang leaves extract 150 mg/kgBW as well as vitamin C 75 mg/kgBW for 30 days. On the 31st day, the mice were killed to check their catalase enzyme activity. In this research, there was a significant (P<0.05) increase in catalase enzyme activity between the K- with K + group and between the K+ group with P4. The conclusion was that jamblang leaves extract was able to increase catalase enzyme activity after being induced by lead acetate.
Keywords: Lead acetate, jamblang leaf extract, vitamin C, catalase enzyme activity, rats.
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