Original Research Article
Year: 2016 | Month: April | Volume: 3 | Issue: 4 | Pages: 18-24
Assessment of Oxidative Stress & Antioxidant Status in Acute Pancreatitis by Measurement of Ischaemia Modified Albumin, Super Oxide Dismutase & Tocopherol
Soumika Biswas1, Pratip Jana2, Arghya Basu3
1Demonstrator, Dept. of Biochemistry, Nrs Medical College & Hospital, Kolkata, West Bengal, India.
2Medical Internee, Nrs Medical College & Hospital, Kolkata, West Bengal, India.
3DNB Dept. of General Surgery, B R Singh Railway Hospital, Sealdah, Kolkata, West Bengal, India.
Corresponding Author: Soumika Biswas
ABSTRACT
Acute pancreatitis (AP) can present either as a mild, self-limiting localized disease to fatal widespread multi-organ failure with high mortality rates. There is increased oxygen free radical production & it causes oxidation of lipids in the pancreatic cell membrane and oxidatively modify proteins, depolarize mitochondrial membrane, and induces DNA fragmentation. In this study, 50 diagnosed acute pancreatitis patients(32 gallstone,15 alcoholic and 3 idiopathic) and age and sex matched healthy controls coming to Dept. of Biochemistry, Medical College and Hospital Kolkata as patient parties between January 2012 to August 2013 were tested to assess the oxidative stress & antioxidant status in acute pancreatitis patients by measurement of ischaemia modified albumin (Albumin Cobalt binding assay) , super oxide dismutase (Kakkar’ method) & tocopherol (Baker & Frank method). The result of this study suggests that there is increased antioxidant status (super oxide dismutase and tocopherol) in healthy controls than acute pancreatitis patients and increased oxidative stress (which is indicated by raised ischemia modified albumin) is present in acute pancreatitis patients. There is scope to elaborate this field with larger sample size, inclusion of different race, and with other oxidative stress parameters.
Keywords: Acute pancreatitis, oxidative stress, super oxide dismutase, ischaemia modified albumin, tocopherol.