Original Research Article
Year: 2016 | Month: September | Volume: 3 | Issue: 9 | Pages: 46-53
Biowaste as Substrate for Laccase Production
Shweta Malhotra, Roselin P
Department of Microbiology, St. Francis College for Women, Begumpet, Hyderabad-500016, Telangana, India.
Corresponding Author: Roselin P
ABSTRACT
Approximately 700 million tons of organic waste such as food waste, livestock manure, vegetable waste and wastewater sludge is produced in India each year. Only 0.5% of these wastes are appropriated as inputs for various processes while the rest is regarded as landfills or dumped. Laccases are multicopper enzymes belonging to the group of blue oxidases. Laccase is widely distributed in higher plants and fungi isolated from soil litter, humus and wood. Potato dextrose media and standard media were used for the production of laccase and the same were incorporated with bio-waste- cauliflower waste and wood apple waste. The production was carried out at 120rpm at 280C for 5 days. The concentration of protein, enzyme activity and % dye decolorisation was measured. From this study we can conclude that media incorporated with bio-waste form potential substrates for production of laccases. Out of the two bio wastes used cauliflower waste and wood apple waste, media incorporated with cauliflower waste showed maximum concentration of protein, enzyme activity and % decolorisation of dye. Media incorporated with both cauliflower waste and wood apple waste also showed a fairly good amount of protein, enzyme activity and % dye decolorisation. The process with optimized fermentation parameters could be used for scaling up of the process to a pilot scale or commercial fermenter level.
Key words: Laccase, Fusarium, screening, biowaste, production.