Original Research Article
Year: 2018 | Month: December | Volume: 5 | Issue: 12 | Pages: 231-238
A Study of Oxidative Stress and Antioxidant Defense in Chronic Kidney Disease in a Tertiary Care Hospital of West Bengal
Sayantan Dasgupta1, Chinmoy Ghosh1, Sandip Chakraborty2, Ishita Mondal3, Satavisa Bhattacharjee4
1Assistant Professor, Department of Biochemistry, NRS Medical College, Kolkata
2Associate Professor, Department of Biochemistry, NRS Medical College, Kolkata
3Demonstrator, Department of Biochemistry, Purulia Government Medical College, Purulia
4Junior Chemist, Envirocheck, Kolkata
Corresponding Author: Chinmoy Ghosh
ABSTRACT
Background: Chronic Kidney Disease (CKD) is one of the major diseases claiming a sizeable fraction of overall mortality and morbidity every year in our country. Multiple studies have found increased oxidative stress and imbalance in antioxidant defense in CKD patients. Apart from the disease pathology itself, other associated factors, like inflammation, hemodialysis etc. also contribute to increased oxidative stress, which in turn complicate the clinical condition further. There are multitude of markers to measure oxidative stress and antioxidant defense, but it is very difficult to measure overall oxidative stress status accurately and completely. There is also very little data available about the oxidative stress status in CKD patients in the population of West Bengal.
Aims and Objectives: This study aims to measure Total oxidative Stress (TOS) and Total antioxidant defense (TAD) status in CKD patients in a sample population of West Bengal, more efficiently, by a colorimetric method modified and standardized in our laboratory. We also aim to find out the relationship (if any) of the Oxidative stress status in these patients with the severity of the disease
Material and Methods: The study included 40 CKD patients from OPD and IPD of a tertiary care hospital of Kolkata, West Bengal, and 30 age and sex matched healthy volunteers. Serum Urea, Creatinine, Sodium and Potassium were measured by standardized commercial kits and TOS and TAD were measured by colorimetric chemical methods, modified and standardized in our laboratory.
Results: The TOS was found to be significantly increased (P=0.003) in CKD patients in comparison to healthy control subjects. TAD was also found to be significantly elevated (P<0.001) in the patient group. However, TOS and TAD showed no correlation with serum creatinine (P>0.05) in our study group.
Conclusion: The total oxidative stress does indeed increase in the chronic Kidney Disease, in the population of West Bengal too, and this result reaffirms the similar findings elsewhere in the world. The increase in Total Antioxidant Defense in our study population, contrary to a usual decrease in TAD found with increase in TOS, indicates a compensatory increase as found and suggested by some previous studies. This study did not find any significant relationship of TOS and TAD levels with disease severity, which warrants a more elaborate study with a larger sample size, and taking into account different confounding factors.
Key words: Total Oxidative Stress, Total Antioxidant Defense, Chronic Kidney Disease
[PDF Full Text]