Original Research Article
Year: 2018 | Month: December | Volume: 5 | Issue: 12 | Pages: 249-253
Developing New Protocol for DNA Isolation Using House Hold Things
Ms. Deepa K P
Assistant Professor, Department of Botany, Bishop Heber College (Autonomous), Tiruchirappalli -620017
ABSTRACT
In this new era of biotechnological world, genetic knowledge is a must to the need and upto the molecular level study has been implemented in the school subjects itself. The DNA isolation and its structural study is an interesting topic to all levels of learning. Molecular study of DNA is available in Higher Education Institutions and it will be new to the young school students. In this context the objective of my study is to develop a new protocol for DNA isolation. The study has conducted to isolate DNA from 6 tropical fruits and the chemicals used were soap oil and ice- cold alcohol. As bromelain has proteinase activity, pine apple is used to serve the purpose of protein digestion. The DNA clumps were observed in 3 fruits clearly, i.e. Strawberry, kiwi, apple and the other fruits like guava, banana has shown little protein impurity. But in grapes, we are unable to isolate the DNA. This study provides new dimension in developing a protocols for DNA isolation using cheap household things and will be more useful for budding students.
Key words: DNA isolation, Bromelain, UV- Spectrophotometer, Methylene Blue, Microscope.
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