Original Research Article
Year: 2018 | Month: April | Volume: 5 | Issue: 4 | Pages: 66-72
Characterization of Pili Protein as Cell Adhesion Molecules of Mycobacterium Tuberculosis H37RV to Mouse Enterocytes: In vitro Study
Dwi Yuni Nur Hidayati1, Dewi Santosaningsih1, Husnul Khotimah2
1Microbiology Laboratory Medical Faculty Brawijaya University
2Pharmacology Laboratory Medical Faculty Brawijaya University
Corresponding Author: Dwi Yuni Nur Hidayati
ABSTRACT
Tuberculosis (TB) is an important public health problem in Indonesia. The number of patients suffering from the disease is the fifth largest in the world. Mycobacterium tuberculosis is a bacterial pathogen that can invade and colonize host cells. The bacterium has pili on its surface that facilitate its adhesion to host cells. Thus, identification and characterization of M. tuberculosis pili proteins can provide insight that may be useful in developing new strategies for the disease control. The aim of present study was to determine hemagglutinins and adhesins in the shaved pili of M. tuberculosisH37rv by using hemagglutination and adhesion tests. This experimental study employed a control group and three observational groups by using a CRD (completely randomized design) approach. The pili of M. tuberculosis were mechanically shaved from the surface by using a pili cutter. The pili proteins were separated by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and were purified by electroelution. The purified proteins were then mixed with mouse erythrocytes to obtain a hemagglutination titer. Following this, adhesion tests were performed to study the ability of pili proteins to attach to mouse enterocytes. It was observed that several pili proteins with molecular weights of 63-kDa, 37-kDa, and 11-kDa were able to attach to mouse enterocytes. A hemagglutination test confirmed that the 11-kDa pili protein was a hemagglutinin. An adhesion test showed that 11-kDa pili protein has the ability to attach on mouseenterocytes. These results were found to be statistically significant using ANOVA (p <0.05). Overall, the data indicate that M. tuberculosis H37Rv produces an 11-kDa pili protein that plays an important role in hemagglutination and adhesion.
Key words: Adhesin protein, Hemagglutinin protein, Mycobacterium tuberculosis strain H37rv.
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