Original Research Article
Year: 2018 | Month: August | Volume: 5 | Issue: 8 | Pages: 177-181
Production, Partial Purification and Assay of L- Glutaminase Enzyme from Aspergillus Niger by Solid State Fermentation
Nagamani J.E
Garden City University, School of Sciences, Bangalore-560 049, Karnataka, India
L-Glutaminase (L-Glutamine amidohydrolase, EC 3.5.1.2) is the important enzyme that catalyzes the deamination of L-Glutamine to L-glutamic acid and ammonium ions. Of late L-Glutaminase has received much attention with respect to its therapeutic and industrial applications. It acts as a potent antileukemic agent and has flavor enhancing property for fermented foods. Glutaminase production is widely distributed in plants, animals, bacteria, yeasts, and fungi. This study investigates the production and partial purification of extracellular Glutaminase enzyme from Aspergillus niger using agricultural waste by solid state fermentation (SSF). 80% ammonium sulphate fractionated enzyme exhibited maximum activity of 10.932µM/ml/min and a specific activity of 0.879U/mg protein.L-Glutaminase (L-Glutamine amidohydrolase, EC 3.5.1.2) is the important enzyme that catalyzes the deamination of L-Glutamine to L-glutamic acid and ammonium ions. Of late L-Glutaminase has received much attention with respect to its therapeutic and industrial applications. It acts as a potent antileukemic agent and has flavor enhancing property for fermented foods. Glutaminase production is widely distributed in plants, animals, bacteria, yeasts, and fungi. This study investigates the production and partial purification of extracellular Glutaminase enzyme from Aspergillus niger using agricultural waste by solid state fermentation(SSF). 80% ammonium sulphate fractionated enzyme exhibited maximum activity of 10.932µM/ml/min and a specific activity of 0.879U/mg protein.
Key words: L-Glutaminase, Aspergillus niger, SSF, Activity, Specific activity
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