Original Research Article
Year: 2019 | Month: October | Volume: 6 | Issue: 10 | Pages: 1-8
Immunohistochemical Characterisation of Basal Like Phenotype of Triple Negative Breast Cancer from Western India
Dr. Hemangini Vora1, Dr. Sonia Parikh2, Dr. Nupur Patel3
1Associate Professor of Cancer Biology, Head of Immunohematology Lab,
2Associate Professor of Medical Oncology, Head of Medical Unit III,
3Research Assistant, Immunohematology Lab,
The Gujarat Cancer & Research Institute, Medicity, Asarwa, Ahmedabad 380016
Corresponding Author: Dr. Sonia Parikh
ABSTRACT
Background and Aim: Triple-negative breast cancer (TNBC), which accounts for approximately 15-20% of all breast cancers, defined as lack of expression of estrogen receptor, progesterone receptor and Her-2 neu receptors. TNBC has two subtypes basal like and non-basal like, the former characterised by aggressive biology with limited therapeutic options. The present study aimed to identify TNBC with basal like phenotype and its correlation with conventional parameters.
Method: This study enrolled 100 untreated TNBC and immunohistochemical localisation of CK5/6, CK14, CK17, EGFR, Vimentin and C-kit was performed on tumor tissues for identification of basal like phenotype. The data was analysed statistically using SPSS software version 20.
Results: CK5/6, CK14, CK17, EGFR, Vimentin and C-kit expression was seen in 36%, 4%, 45%, 45%, 50% and 38% TNBC, respectively. Of 100 TNBC patients, 77% were positive for basal marker expression identified as basal like and 23% were negative for basal marker expression identified as Non-Basal. Further, multivariate analysis by General Linear Model indicated that four markers combination (vimentin, EGFR, CK17, CK5/6) has maximum 1.00 observed power of the test for identification of Basal-like TNBCs. In univariate and multivariate survival analyses, none of the marker discriminated patients with worse or better relapse free survival.
Conclusion: A panel of markers includes Vimentin, EGFR, CK17 and CK5/6 can be used for classification of basal like TNBCs.
Key words: TNBC, Basal like, Immunohistochemistry markers
[PDF Full Text]