Original Research Article
Year: 2019 | Month: October | Volume: 6 | Issue: 10 | Pages: 321-331
Immunohistochemical and Morphometric Study of Urinary Bladder Epithelial Lesions with Special References to Invasiveness and Proliferative Activity
Dr. Bappa Mandal1, Dr. Sucharita Sarkar2, Dr. Asim Kumar Manna3, Dr. Saswati Sengupta4, Dr. Mousumi Bag4
1Assistant Professor, Institute of Post Graduate Medical Education and Research,;Neonatal unit, Department of Pathology , Kolkata-700 020, West Bengal
2Demonstrator, R.G Kar Medical College & Hospital, Kolkata - 700004, West Bengal.
3Professor, Institute of Post Graduate Medical Education and Research, Kolkata-700 020, West Bengal
4Post graduate trainee,Dept of Pathology, Institute of Post Graduate Medical Education and Research, Kolkata-700 020, West Bengal
Corresponding Author: Dr. Sucharita Sarkar
ABSTRACT
Context (background) & aims – The urinary bladder is a part of lower urinary tract, one of the main portals of the urogenital system. The different bladder lesions constitute a major health problem in developing countries. Carcinoma of this region is the commonest neoplasm in elderly male in our country. Our study was done with the aim of early diagnosis of these lesions in a cost effective manner.
Materials & Methods - Total 62 cases were selected having signs and symptoms of lower urinary tract infection and hematuria. The specimens were collected from the Urology department of a tertiary care hospital situated at Kolkata. Thereafter, the tissues were processed routinely for histopathology and immunohistochemistry (p53, ki-67 & 34βE12 cytokeratin).Morphometric analysis of Mean Nuclear Diameter (MND), Mean cytoplasmic diameter (MCD), Mean nuclear area (MNA), Mean Nuclear Perimeter (MNP), Nucleo-cytoplasmic ratio (N: C) etc were done on H & E stained sections with ERMA ocular micrometer and analysed by software AutoCAD 2007.
Statistical analysis used - Unpaired Student’s t-Test.
Results - Statistically significant difference was found between non-neoplastic and malignant lesions. The diagnosis was confirmed by histopathological findings along with morphometry and proliferative activity as well as invasiveness were assessed with ki-67 and 34βE12 cytokeratin immunolabelling.
Conclusion - Morphometry can be an important tool for early diagnosis and determination of therapeutic protocol in the different urinary bladder lesions especially if they are correlated with immunohistochemistry.
Key words: Urinary bladder lesion, Morphometry, Immunohistochemistry, ki-67.
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