Original Research Article
Year: 2019 | Month: November | Volume: 6 | Issue: 11 | Pages: 275-284
Histone Methylation Befuddledness in the Infralimbic Prefrontal Cortex and Their Association with Extinction Memory
Rohit Kanojia1, Dinesh Raj Modi2, Suman Mishra1, Kamal Jaiswal1
1Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow-226025, India.
2Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow-226025, India.
Corresponding Author: Kamal Jaiswal
ABSTRACT
The limited neurological understanding especially the molecular mechanism involved in fear extinction has been attributed to the need for improved animal models for the treatment of anxiety disorders. We presently hypothesized that the mechanism, how timing of fear extinction for a specific fear affect the histone methylation and their effect in retracement. Fear memory acquisition followed by extension training was given to rats for 10 minutes which deficits in the retention of extinction memory when compared to the other which went for 24 hours of extinction after fear acquisition. The first one is immediate extinction (IE) and the second is delayed extinction (DE). We analyzed that the activity of infralimbic prefrontal cortex (IL) to prelimbic cortex (PL) was decreased in IE when compared to DE and confounded with the activity and expression of c-fos in mPFC. As a confirmation we further analyzed the acetylation of histone H3/H4 and levels of CREB binding protein (CBP) which is a histone acetyltransferase (HAT) and found that this was associated with the activation of neuron and is significantly decreased in IL of IE as compared to the DE. We finally conclude that the deficits in IE is mainly due to the sustained activation of IL because of it is associated with the changes involved in histone methylation.
Key words: Fear memory, histone acetyltransferase, histone methylation, CREB binding protein, prelimbic cortex
[PDF Full Text]