Review Article
Year: 2019 | Month: February | Volume: 6 | Issue: 2 | Pages: 170-179
PCR- Revolutionizing the Diagnostic Approach and Applications in Dentistry: A Review
Pranita A. Rode1, Rajashri A. Kolte2, Abhay P. Kolte3
1Lecturer, 2Professor, 3Professor & Head
Department of Periodontics and Implantology, VSPM Dental College and Research Centre, Nagpur.
ABSTRACT
The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. Three major steps involved in the PCR technique: denaturation, annealing, and extension. Qualitative PCR can be used to detect not only human genes but also genes of bacteria and viruses, thus useful in the investigation and diagnosis of a growing number of diseases. Various types of PCR are applicable in dental diagnostic procedures. Articles from the esteemed journals are referred to review principles, applications, advantages and disadvantages of PCR in laboratory diagnosis of disease in this article.
Key words: Polymerase Chain Reaction, Molecular techniques, DNA amplification.
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