Original Research Article
Year: 2019 | Month: March | Volume: 6 | Issue: 3 | Pages: 147-154
Assessment of Chromosomal Abnormalities in Couples with Recurrent Pregnancy Loss
Dr. Sujithaa Nagarajan1, Dr. Suma H Y2, Dr. Latha Chaturvedula3
1Assistant Professor, Department of Anatomy, Government Villupuram Medical College & Hospital, Villupuram, India.
2Additional Professor, Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Dhanvantri Nagar, Pondicherry, India.
3Professor, Department of Obstetrics & Gynecology, Jawaharlal Institute of Postgraduate Medical Education and Research [JIPMER], Dhanvantri Nagar, Pondicherry, India.
Corresponding Author: Dr. Sujithaa Nagarajan
ABSTRACT
Previous studies have demonstrated the type of chromosomal abnormalities found among couples with recurrent pregnancy loss. But a majority of the couples show a normal karyotype. The present study was undertaken to inquire the nature of cytogenetic abnormalities by routine karyotyping and c-banding whenever required, among couples with recurrent pregnancy loss and to compare it with fertile couples. Among couples, women with the history of two or more than two spontaneous abortions ≤ 24 weeks of gestation were included and couples with the history of Diabetes mellitus, thyroid disorders and hypertension, etc. were excluded. The lymphocytes were cultured as per the standard protocol and metaphase spreads stained by GTG banding technique. The presence of chromosomal aberrations was analyzed by semi-automated karyotyping software (Ikaros). In the present study, abnormal karyotypes were found in 3 cases. All the controls had a normal karyotype.
Key words: Chromosomal abnormalities, Karyotyping, Recurrent pregnancy loss, C-banding, Heteromorphisms.
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