Original Research Article
Year: 2019 | Month: July | Volume: 6 | Issue: 7 | Pages: 169-174
Study of Gram Negative Non Fermenting Bacilli from Surgical Site Infections in a Tertiary Care Hospital in Kolkata
Dr. Sonia Deb1, Dr. Soma Sarkar2, Dr. Chitrita Chatterjee3, Dr. Nishith Kumar Pal4, Dr. Swagata Ganguly5
1Junior Resident, 2Associate Professor, 3Professor and Head of Department, 4Professor and Ex- Head of Department,
Department of Microbiology, N.R.S. Medical College and Hospital, Kolkata
5Associate Professor, N.R.S. Medical College and Hospital, Kolkata
Corresponding Author: Dr. Swagata Ganguly
ABSTRACT
Surgical site infections (SSI) result in increase in hospital stay and strain on hospital economy. Increasing number of Non-fermentative Gram-negative bacilli (NFGNB) isolates from SSI which are frequently multi drug resistant (MDR), necessitate proper identification and susceptibility pattern for effective management. The aim of this study was to determine the point prevalence of SSI by NFGNBs and to assess their susceptibility pattern to antimicrobials. A prospective study of 300 cases of SSI was studied from pus samples received in the Department of Microbiology, N.R.S.M.C.H over a period of six months (Jan 2018 to June 2018). These clinical samples were subjected to classical bacteriological diagnostics and NFGNB were identified using a standard protocol. Antimicrobial susceptibility testing was done by Kirby Bauer disc diffusion method. Out of 300 SSI cases, significantly 89 (29.6%) isolates were NFGNB. Genus and species wise predominant isolates were Pseudomonas aeruginosa (52.8%) and Acinetobacter baumannii (39.3%); others were Stenotrophomonas maltophilia (3.37%), Acinetobacter lwoffii (2.24%), Pseudomonas fluorescence (1.12%) and Sphingomonas paucimobilis (1.12%). All Acinetobacter baumannii isolates could multiply at 10⁰C which is a significant collateral finding. Imipenem resistance was noted in 82% Acinetobacter baumannii and 9% Pseudomonas aeruginosa, whereas 76% Acinetobacter baumannii and 5% Pseudomonas aeruginosa were resistant to meropenem. Colistin resistance was found in 2.85% Acinetobacter baumannii isolates. All 3 Stenotrophomonas maltophilia were resistant to Levofloxacin and Cotrimoxazole. Our study showed high number of MDR NFGNB causing SSI. As mostly NFGNB infections are exogenous, this study highlights importance of following strict aseptic measures to control such infections.
Key words: NFGNB, SSI
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