Original Research Article
Year: 2019 | Month: August | Volume: 6 | Issue: 8 | Pages: 184-194
Comparative Evaluation of Antibacterial Activity of Punica granatum, Acacia nilotica and Emblica officinalis against Enterococcus faecalis and Their Smear Layer Removal Ability When Used as Endodontic Irrigants: an In-Vitro Study
Dr. Payal A Jain1, Dr. Sunil Tejaswi2, Dr. Parinitha M.S2, Dr. Suneeth Shetty3, Dr. Ambikathanaya U.K3,
1Post Graduate Student, 2Reader, 3Lecturer,
Department of Conservative Dentistry and Endodontics, JSS Dental College and Hospital, JSS Academy of Higher Education and Research, JSS Medical Institutions Campus, Bannimantap, Sri Shivarathreeshwara Nagara, Mysuru – 570015, Karnataka, India.
Corresponding Author: Dr. Payal A Jain
ABSTRACT
Context: Phytotherapeutic agents as alternatives for synthetic endodontic irrigants, due to the adverse effects of synthetic agents.
Aims: Aim of this in -vitro study is evaluate and compare antibacterial activity against E.faecalis and smear layer removal efficacy of Punica granatum, Acacia nilotica and Emblica officinalis distilled water extracts.
Methods and Material: Distilled water extracts of the three herbal products were prepared. Minimum inhibitory concentration was determined using Broth microdilution method. Agar well diffusion test was performed and zones of inhibition were measured to evaluate the antibacterial activity.
Sixty human maxillary incisors were decoronated, divided into six groups (n=10).
Group 1: 0.9% normal saline; Group 2: 1% sodium hypochlorite; Group 3: 17% ethylenediaminetetraacetic acid; Group 4: 6.25% Punica granatum; Group 5: 25% Acacia nilotica; Group 6: 12.5% Emblica officinalis solution. Teeth were split into two halves and observed under scanning electron microscope to analyse the amount of smear layer present.
Results: Minimum inhibitory concentrations of Punica granatum, Acacia nilotica and Emblica officinalis were recorded as 6.25%, 25% and 12.5% respectively, with mean inhibition zones of 21mm, 18mm and 20mm. Group 4 showed least smear layer scores, followed by Group 6 and Group 3.
Conclusions: Punica granatum and Emblica officinalis aqueous extracts showed effective antibacterial activity and smear layer removal efficacy in all parts of root canal.
Key words: Acacia nilotica; antibacterial activity; Emblica officinalis; Enterococcus faecalis; Punica granatum; smear layer.
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