Original Research Article
Year: 2020 | Month: November | Volume: 7 | Issue: 11 | Pages: 408-411
A Comparative Evaluation of Microscopic and Immuno-Chromatographic Diagnostic Tests for Detection of Malaria
Harjinder Singh1, Swati Mittal2, Lovepreet Singh3, Ravi Kumar Tiwary4
1M. Sc. Student, Department of Microbiology, Adesh Institute of Medical Sciences and Research, Bathinda.
2Assistant Professor, Department of Microbiology, Adesh Medical College and Hospital, Shahabad (M).
3M. Sc. Student, Department of Microbiology, Adesh Institute of Medical Sciences and Research, Bathinda.
4Consultant, Department of Neurosurgery, Adesh Medical College and Hospital, Shahabad (M).
Corresponding Author: Swati Mittal
ABSTRACT
The diagnosis of Malaria largely depends on clinical judgment, microscopy and recently by rapid immuno-chromatographic diagnostic tests. The present study was conducted in the Department of Microbiology, Parasitology Laboratory, AIMSR (Bathinda). The main objectives of the study were detection and identification of malarial parasite microscopically in blood films and malarial antigen by immuno-chromatographic Rapid diagnostic test. Comparison of the results of microscopic and immuno-chromatographic rapid diagnostic test was then made. Out of 82 blood samples, 7.31% (6) samples were found positive for malarial parasite by microscopy and. 6.09% (5) were found positive by immuno-chromatographic test. Equal number of P. falciparum was detected in blood smears and Immuno-chromatographic tests but detection of P. vivax by blood smears was more as compared to Immuno-chromatographic tests. The comparative analysis thus concludes that though, immuno-chromatographic tests are rapid, do not require expertise and are useful in routine diagnosis, their sensitivity of antigen detection test in lower (97.4%), when compared to microscopy.
Keywords: Immuno-chromatographic test, Microscopy, Jaswant Singh Bhattacharji stain, Malarial parasite.
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