Research Paper
Year: 2020 | Month: January | Volume: 7 | Issue: 1 | Pages: 159-163
Antibacterial Activity Test of Lactobacillus plantarum Bacteriocin Isolated from Dadiah
Sura Aisyah Tampubolon1, Netti Suharti2, Edison3, Andani Eka Putra2
1Master of Biomedical Science Program, Faculty of Medicine, Universitas Andalas, Padang City, Indonesia
2Department of Microbiology, Faculty of Medicine, Universitas Andalas, Padang City, Indonesia
3Department of Public Health and Community Medicine, Faculty of Medicine, Universitas Andalas, Padang City, Indonesia
Corresponding Author: Sura Aisyah Tampubolon
ABSTRACT
Background: Dadiah is a traditional food in West Sumatra, Indonesia which is obtained from the buffalo milk fermentation process. The aim of this study to determine antibacterial activity test of Lactobacillus plantarum bacteriosin insulated from dadiah.
Methods: This research was an experimental of completely randomized design with determined minimum inhibitory concentration and minimum bactericidal concentration which determined by using dilution method in some concentrations of 100%, 80%, 60%, 40%, 20%, and 0%. Identification of bacteriocin from Lactobacillus plantarum isolates was carried out using the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method.
Results: The results of the study known that percentage of Bacteriocin of minimum inhibitory concentration from Lactobacillus plantarum against P. aeruginosa was 98.57% (p<0.001). Meanwhile, the results of minimum bactericidal concentration cannot be identified. Our result showed that the bacteriocin protein band was in the range of 17-28 kDa.
Conclusion: This analysis confirmed that the bacteriocin compound from Lactobacillus plantarum had a bacteriostatic effect on P. aeruginosa.
Keywords: Dadiah, Lactobacillus plantarum, Bacteriocin, Pseudomonas aeruginosa.
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