Research Paper
Year: 2020 | Month: March | Volume: 7 | Issue: 3 | Pages: 488-494
The Effect of Gambir’s Catechin Isolate (Uncaria Gambir Roxb.) on Alanine Aminotransferase and Aspartate Aminotransferase Serum Level at Male Rats (Rattus norvegicus) Wistar Strain Induced by High Fat Diet
Sari Almira Taria1, Rauza Sukma Rita2, Afriwardi3
1Master Program of Biomedical Sciences, Faculty of Medicine, Andalas University, Padang, Indonesia
2Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia
3Department of Physiology, Faculty of Medicine, Andalas University, Padang, Indonesia
Corresponding Author: Rauza Sukma Rita
ABSTRACT
Background: High fat diet causes inflammation of the liver due to accumulation of fat in the liver cells. Catechin isolates of gambir (Uncaria gambir roxb) are an active substance contained in gambir plants which contain flavonoid that have antioxidants activity and potentially as hepatoprotector. This study aims to determine the effect of catechin isolates on alanine aminotransferase (ALT) and aspartate aminotransferase (AST) serum levels at male rats (Rattus norvegicus) wistar strain induced by a high fat diet.
Research Method: This study was true experimental study with post-test only control group design. We use catechin isolates from gambir which are consisting (+)- catechin. This study was conducted on 30 rats divided into 5 groups, ie, negative control group (K-), positive control (K +) and three treatment groups (P1, P2, P3). The K+, and the three treatment groups were induced by high fat diet. The treatment groups received 10 mg/kgBW/day, 20 mg/kgBW/day, and 40 mg/kgBW/day on P1, P2, and P3 groups of catechin isolate of gambir for 14 days. AST and ALT levels were examined using IFCC method. Data analysis was performed using One way Annova and Post Hoc LSD.
Research Result: High fat diet induction could increase AST and ALT serum on the positive control group compared to the negative control group. There was significant difference in AST and ALT levels after being given by catechin isolates between P3 (40 mg/kgBW/day) and K+. The average level of AST and ALT serum for K- was 26.51 U/L;31.58 U/L, K+ was 38.31 U/L; 67.45 U/L, P1 was 35.90 U/L; 66.35 U/L, P2 was 31.13 U/L; 59.71 U/L, and P3 was 30.36 U/L; 38.21 U/L. The differences of AST levels in each groups were significant with p value=0,02 and ALT levels as well, p value=0.001 (p<0.05).
Research Conclusion: The conclusion of this study is catechin isolates of gambir can decrease AST and ALT serum levels.
Keywords: gambir, catechin isolate, AST, ALT, high fat diet
[PDF Full Text]