Original Research Article
Year: 2020 | Month: April | Volume: 7 | Issue: 4 | Pages: 22-29
Biodegradation of Textile Dye by Ligninolytic Bacteria Isolated From Western Ghats
Johnson Elizabeth Mary, Thiruppathi Krithika, Rangasamy Kavitha
Department of Biochemistry, Bharathiar University, Coimbatore, Tamil Nadu, India - 641046.
Corresponding Author: Rangasamy Kavitha
ABSTRACT
Improper disposal of synthetic dyes in waste water causes severe environmental problems. The dye methylene blue is used in various industries and it causes several mutagenic and toxicological effects in living organisms on its exposure. Bioremediation is an emerging technique for effluent treatment. In our study, we aimed to isolate Methylene blue degrading ligninolytic bacteria originated from Western Ghats soil. Totally twenty-one bacterial strains were isolated and among them, nine strains were found to be ligninolytic and able to decolorizing methylene blue. Potential strain with maximum methylene blue decolorizing efficiency was selected and identified by 16S rRNA gene sequencing studies. Influence of environmental parameters such as pH, initial dye concentrations and the mechanism of dye removal were studied with the selected strain. The bacterial strain B1 have the ability to decolorizes up to 71% in nutrient media containing methylene blue and was selected as potent strain and identified as Bacillus cereus with 100% similarity. Maximum decolorizing efficiency was found to be in a nutrient medium containing up to 50mg/l of MB dye at pH 5. The isolated ligninolytic bacterium Bacillus cereus WGB1 has good potential for the degradation of methylene blue and can be applied at textile industry for the treatment of textile dye wastewater for environmental safety.
Keywords: Ligninolytic bacteria, biodegradation, FTIR, Bacillus cereus WGB1, Methylene blue
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