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Original Research Article

Year: 2020 | Month: April | Volume: 7 | Issue: 4 | Pages: 553-559

Comparison of Microscopic Methods with CBNAAT in Suspected Pulmonary Tuberculosis Patients among HIV Seropositive

Ashwini B. S¹, Anuradha K²

¹ Post Graduate, Department of Microbiology, MMC&RI, Mysore
¹Assistant Professor, Department of Microbiology, Sri Siddhartha Medical College, Tumkur
²Professor and Head, Department of Microbiology, MMC&RI, Mysore

Corresponding Author: Anuradha K

ABSTRACT

Introduction: Tuberculosis is the most common opportunistic infection in people with HIV. The two pathogens namely Mycobacterium tuberculosis and HIV potentiate one another, accelerating immunological deterioration and causing premature death if untreated. Accurate diagnosis of disease remains the biggest obstacles to global tuberculosis control. Direct sputum smear microscopy is the cornerstone of tuberculosis diagnosis worldwide. RNTCP is currently using CBNAAT to diagnose tuberculosis and to find out rifampicin resistance.
Objective: To compare the conventional microscopic techniques with CBNAAT for early diagnosis and treatment of co-infection patients.
Methods: This study involved 220 HIV seropositive with symptoms suggestive of tuberculosis. Spot and early morning sputum samples were collected and were subjected to Z-N staining, fluorescent staining (before and after adding sample reagent for the purpose of homogenization) and CBNAAT.
Results: Among these 220 patients, HIV-TB coinfection was more common in males between 31-40 years with CD4 cell count less than 200 cells/µl. out of 220 sputum samples, 29(13%) samples were positive by CBNAAT, among them 15 by fluorescent staining whereas, only one sample was positive for Mycobacterium tuberculosis by all three methods.
Conclusion: CBNAAT was found to be better method compared to conventional microscopic methods and among the microscopic methods, fluorescence staining is better technique.

Keywords: Mycobacterium tuberculosis, HIV, HIV-TB coinfection, Z-N staining, fluorescent staining, CBNAAT

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