Original Research Article
Year: 2020 | Month: June | Volume: 7 | Issue: 6 | Pages: 129-133
Deep Vein Thrombosis- Role of Platelet Derived Micro particles in Coagulation
Satyendra K. Tiwary1, Anand Kumar Das2, D. Das3, Puneet Kumar3, Ajay K Khanna3
1Associate Professor, 3Professor
Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi.
2Resident at Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi
Corresponding Author: Satyendra K. Tiwary
ABSTRACT
Introduction: Several parameters may help in identification of DVT. Though the most important diagnosis of DVT is by compression Ultrasound but there are several other parameters as D Dimer, P-selectin which may help in identification of DVT. Platelets are one of the important factor which may be altered in patients with DVT. We wanted to look into the status of Platelet Derived Micro particles (PDMP),
Methods: A total of 10 patients and 10 controls were included in the study. We included all acute DVT patients as confirmed by duplex scan and excluded those patients who were on antiplatelet or anticoagulants.
Isolation of platelet derived microparticles was done after blood sampling with 2 ml of blood collected in blue top sodium citrate vials from each case and control were sent to Biochemistry laboratory and studied within 1-2 hrs.Agonist induced conformational change in integrin αβ results in high affinity binding of fibrinogen to platelet surface. PAC-1 antibody specifically recognizes open conformation of αβ. Therefore, PAC-1 antibody was used to study the effect of agonist on integrin activation.
Results: Among all 10patients (6 males and 4 females). 8 patients were in age group 20-39 years. Platelet derived microparticles are 3-4 times raised as compared to healthy individual. Median number of platelet derived microparticles of all cases was 4461.5/105 platelet cells and median number of platelet derived microparticles of all controls was 1868.0/105 platelet cells.
Conclusion: Mean number of PDMPs in each case with control which showed that mean number of microparticles were significantly raised in cases (4.09±1.2 vs 3.06±0.5; p=0.029).
Keywords: DVT, Platelets, Micro particles, Micro vesicles, Platelet Derived Micro particles (PDMP)
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