Research Paper
Year: 2020 | Month: July | Volume: 7 | Issue: 7 | Pages: 384-389
Manufacture and Characterization of Microcrystalline Cellulose of Water Hyacinth (Eichhornia Crassipes (Mart.) Solms) By Enzymatic Process
Rina Desni Yetti, Zulharmita, Rega Junita
Sekolah Tinggi Ilmu Farmasi (STIFARM) Padang, Indonesia
Corresponding Author: Rina Desni Yetti
ABSTRACT
Research on manufacture and characterization of microcrystalline cellulose from water hyacinth (Eichhornia crassipes (Mart.) Solms) has been carried out enzymatically using cellulase enzymes produced from Trichoderma Viride fungi. The water hyacinth leaves are cut small and washed and then dried. The dried water hyacinth leaves were mashed and the water hyacinth powder was obtained then macerated with 2 liters of ethanol for 24 hours. Then maceration powder was extracted with 17.5% NaOH for 5 hours, then filtered and washed with distilled water. After it was dried in the oven and then obtained alpha cellulose. The alpha cellulose obtained was then hydrolyzed enzymatically using the cellulase enzyme so that the hydrolysis results were microcrystalline cellulose compounds. The resulting MCC was tested for FTIR, SEM, DSC. Based on the results of the study obtained the results of the FTIR test obtained functional groups O-H, C-H, C = C-H. Same with the comparison of Vivacel PH 102. The SEM results are looking at the surface morphology of a sample with a magnification of 1000 obtained a crystal form. The results of the Vivacel DSC thermogram PH 102 are melting points (85.406oC). MCC that was made was obtained the average melting point (93.196oC). The results showed that water hyacinth has the potential to show characterization similar to the reference microcrystalline cellulose (Vivacel PH 102).
Keywords: Microcrystalline Cellulose, Water Hyacinth, Cellulase Enzyme.
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