Research Paper
Year: 2021 | Month: September | Volume: 8 | Issue: 9 | Pages: 18-22
DOI: https://doi.org/10.52403/ijrr.20210904
Effect of Streptozotocin on Liver Histology Damage in Rats Model of Gestational Diabetes Mellitus
Liganda Endo Mahata1, Hirowati Ali2, Arina Widya Murni3
1Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Andalas, Indonesia
2Department of Biochemistry, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
3Department of Internal Medicine, Faculty of Medicine, Universitas Andalas, Padang, Indonesia
Corresponding Author: Liganda Endo Mahata
ABSTRACT
Background: Gestational Diabetes Mellitus (GDM) is a disorder of carbohydrate metabolism that causes hyperglycemia, insulin resistance and failure of organs especially the liver. There is great interest in understanding the pathophysiology and treatment of GDM. Due to ethical issues involving human studies, it is necessary to use animal models to understand pathophysiology and potential treatment for GDM. Streptozotocin-induced diabetes mellitus in pregnant rats was commonly used by several author.
Aims: The aim of this study is to investigate the effect of streptozotocin (STZ) on liver histology in pregnant rats.
Methods: Pregnant rats were divided into two groups; 1) Negative control, 2) Positive control. Positive control were pregnant rats induced with a single intraperitoneal injection of streptozotocin 40 mg/Kg b.w. Fourteen days after induction, rats were sacrificed to evaluate the histopathological effect of STZ on the liver using hematoxylin Eosin staining and calculate the presentation of degraded cell and sinusoidal area with ImageJ 1.49v software, National Institute of Health, Bethesda, MD, USA. Data were processed statistically using SPSS with T-Test.
Results: Microscopic examination of the liver of STZ-induced rats showed histologic changes in the form of an increase in the number of degenerated cells and a significant expansion of the sinusoidal area (p < 0.000). The percentage of degenerated cells in the healthy group was 9.3%, increased to 70% in the STZ-induced group. In addition, the percentage of the sinusoidal area, which was 19.98% in the healthy group, increased to 49.5%.
Conclusions: Streptozotocin induces liver damage in the pregnant rats model.
Keywords: Gestational Diabetes Mellitus, Streptozotocin, Liver, Histology
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