Research Article
Year: 2022 | Month: April | Volume: 9 | Issue: 4 | Pages: 1-5
DOI: https://doi.org/10.52403/ijrr.20220401
Differences of IL-12 Levels between Positive and Negative Helicobacter Pylori Gastritis
Gontar Alamsyah Siregar1, Zaimah Z Tala2
1Division of Gastroenterohepatology, Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
2Department of Nutrition, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Corresponding Author: Gontar Alamsyah Siregar
What is Important?
IL-12 is one of several inflammatory cytokines in response to bacterial infection. In gastritis patients, a significant increase in IL-12 was reported. A significant difference between IL-12 levels in gastritis patients with H. pylori was seen compared with gastritis without H. pylori.
ABSTRACT
Introduction: Gastritis is inflammation of the stomach that can occur either acutely or gradually as a chronic condition. The incidence of gastritis in Indonesia reaches 40.8%. One of the most common causes of gastritis is Helicobacter pylori (H. pylori). Gastric mucosal damage due to inflammation will induce natural body defense cells and increase inflammatory cytokines, one of which is IL-12.
Methods: The study was conducted on 80 samples obtained through consecutive sampling in March-June 2019. Gastritis was diagnosed through endoscopic examination (Olympus, Tokyo, Japan). H. pylori was determined by a change in color to red, magenta, pink or orange on Campylobacter Like Organism (CLO) examination. IL-12 level was determined using a Human IL-12 ELISA kit (ab46035) | Abcam. All data were collected and analyzed with SPSS version 23.
Results: A total of 80 subjects were enrolled with male predominant. The mean of Body Mass Index (BMI) was 23.16 kg/m2 and the mean of total IL-12 levels was 49.38 + 19.71 pg/mL. IL-12 levels were significantly higher in positive H. pylori gastritis (65.36 + 16.58 pg/mL) than negative H. pylori gastritis (40.77+15.5 pg/ml), p <0.001.
Conclusion: There was a significant difference between IL-12 levels in patients with H. pylori gastritis compared with gastritis without H. pylori.
Keywords: IL-12, Gastritis, Helicobacter Pylori.
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