Thesis
Characterization of Optical Biopsy (OCT Images) of Oral Squamous Cell Carcinoma (OSCC) and Its Correlation with Histopathological Attributes
Dr. Snehanjan Sarangi1, Prof. (Dr.) R.R. Paul2, Prof. (Dr.) M. Pal3, Dr. J. Chatterjee4
1Post Graduate Student, Department of Oral and Maxillofacial Pathology, Guru Nanak Institute of Dental Sciences and Research, Panihati, Kolkata.
2Guru Nanak Institute of Dental Sciences and Research, Panihati, Kolkata.
3Guru Nanak Institute of Dental Sciences and Research, Panihati, Kolkata.
4Guru Nanak Institute of Dental Sciences and Research, Panihati, Kolkata.
Corresponding Author: Dr. Snehanjan Sarangi
ABSTRACT
The present study had been conducted on a total number of six subjects in Guru Nanak Institute of Dental Sciences and Research and SMST, IIT, Kharagpur during the time period of December 2014- August 2015 for characterization of Optical biopsy (OCT images) of Oral squamous cell carcinoma (OSCC) and its correlation with histopathological attributes, to establish the efficacy of Optical coherence tomography(OCT) as an adjunctive non invasive diagnostic imaging modality, for an efficient and painless diagnosis Oral squamous cell carcinoma.
The study subjects were thoroughly interrogated, examined, assessed and all the analysed relevant observations were recorded carefully. Then real time in vivo OCT imaging from the representative areas of the lesion with prior informed consent was performed. Thereafter, incisional biopsies were performed from the exact site and the biopsied specimens were subjected to an ex vivo OCT imaging protocol after a time lapse of six hours, which was the time required for transportation of the tissues to SMST. Afterwards, the biopsied samples were histopathologically evaluated, for the presence of corroborative architectural analytical parameters, with the OCT images.
OCT images appear as varying areas of hyper/hypo lucid zones depending upon the area through which the light is backscattered or transmitted. Hyper lucid zones appear as brighter areas and hypo lucid zone appears as darker areas.
Optical coherence tomography is an evolving imaging technology and a non invasive imaging technique for evaluating oral structures. It can be used for the diagnosis of oral premalignant and malignant changes in the oral mucosa. Because of its higher resolution and penetration depth it can be used for imaging the normal and abnormal changes in the oral mucosa ,but a lot of research is required still, to establish OCT as a non invasive diagnostic imaging modality which might be a replacement for gold standard histopathology. As it is a new and emerging technology in oral health sciences, several ongoing studies can provide relevant information regarding the use of OCT, which might be of routine diagnostic value. It can be said that the performed study is a step in the right direction in this regard, as it might do value additions to this exciting and innovative bio-engineering research arena and enrich future researchers to pursue further studies. However, the SS –OCT cannot discern cellular details. So, a higher resolution OCT, such as the HD-OCT can be useful in identifying cellular details. Hence, studies involving increased number of samples would be quite helpful in establishing the credentials of OCT as a non invasive alternate imaging modality for screening and diagnostic purposes. Further studies may improve the resolution of the system and the probe capabilities which shall provide valuable information on the development and progression oral precancers and cancers.
Keywords: Optical Biopsy (OCT Images), Oral Squamous Cell Carcinoma (OSCC), Histopathological Attributes
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